Method for tracing nerve fiber bundles in insect light color tentacle

The invention discloses a method for tracing nerve fiber bundles in an insect light color tentacle, and belongs to the technical field of experimental sample processing. The tracing of the nerve fiberbundle in the intact tentacle can be realized through living tentacle nickel chloride or cobalt chloride immersion, living tentacle and brain microseparation, color development, fixation, dehydration, tentacle and brain transparent treatment, tentacle posture fixation and microphotography. The problem of intact tentacle transparency and multi-angle visualization of the nerve fiber bundles under the condition of no slicing or destroying the tentacle can be solved and the connection of the nerve fiber bundles and the brain in the tentacle can be directly observed at the level of nerve pathway.The method is low in cost, reliable, easy and convenient to operate and good in reproducibility and can directly obtain the multidimensional data of the internal neural structure of the tentacle. 本发明公开了一种昆虫浅色触角内神经纤维束示踪的方法，属于实验