Constructing method of model for studying enhancement of seleno-glycosaminoglycan on immunologic mechanism of black porgy based on spleen metabonomics

The method, which relates to the biological medicine analysis field, provides a constructing method of a model for studying enhancement of seleno-glycosaminoglycan on an immunologic mechanism of the black porgy based on spleen metabonomics. The method comprises: step one classifying juvenile fishes of the black porgy into an experimental group and a blank group; step two, adding seleno-glycosaminoglycan into fodder of the experimental group; step three, after foddering ending, carrying out starvation treatment on juvenile fishes, taking out spleen tissues after anesthetization, and storing thespleen tissues for standby; step four, preparing a spleen tissue sample and a quality control sample, and carrying processing by using a ultra-high performance liquid chromatography-time-of-flight mass spectrometry joint technology; and step five, processing collected metabolomics data, identifying and screening a spleen metabonomics biomarker for enhancement of seleno-glycosaminoglycan on an immunologic mechanism of the