Primers, probe group, kit and detecting method for duplex detecting of Gardner's bacillus and trichomonas vaginalis

The invention relates to the technical field of detection of pathogenic microorganisms, in particular to primers, a probe group, a kit and a detecting method for duplex detecting of Gardner's bacillusand trichomonas vaginalis. The kit comprises GD/TV conserved domain specific primer pairs and T...

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Bibliographische Detailangaben
Hauptverfasser:	WEI YINGYING, SONG GAOSHANG, GUO GUANGHUA, WU QIAN, SHEN JIANGWEI, ZOU GUOBAO, LIN HAO, LIU XINXIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the technical field of detection of pathogenic microorganisms, in particular to primers, a probe group, a kit and a detecting method for duplex detecting of Gardner's bacillusand trichomonas vaginalis. The kit comprises GD/TV conserved domain specific primer pairs and Taqman fluorescent probes. Through a real-time fluorescent quantitative PCR, the kit can accurately detect GD/TV infection samples and is high in sensitivity, specificity and repeatability and convenient and rapid to use. 本发明涉及病原微生物的检测技术领域，尤其涉及双重检测加德纳杆菌和阴道毛滴虫的引物、探针组、试剂盒及检测方法。所述试剂盒中包含GD/TV保守区域特异的引物对和Taqman荧光探针，该试剂盒通过实时荧光定量PCR，能准确地将GD/TV感染样本检测出来，灵敏度高、特异性好、重复性强，且使用方便快捷。