Method for extracting exosomes and exosome proteins

The invention discloses a method for extracting exosomes and exosome proteins from human body fluid or culture medium. The method for extracting exosomes and exosome proteins provided by the inventionincludes: extraction materials are added to to-be-extracted solution containing the exosomes, and ca...

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Hauptverfasser: QIAN XIAOHONG, ZHANG YANGJUN, JIAO FENGLONG, WU QIONG, XIA CHAOSHUANG, LYU YAYAO, GAO FANGYUAN, YU QIAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for extracting exosomes and exosome proteins from human body fluid or culture medium. The method for extracting exosomes and exosome proteins provided by the inventionincludes: extraction materials are added to to-be-extracted solution containing the exosomes, and carrying out extraction to obtain the exosomes; the extraction materials are metallic oxides or function materials modified with the metallic oxides; the metallic oxides and phosphate anions can occur double coordination; and the to-be-extracted solution is body fluid from the human body, diluent ofthe body fluid or cell culture products. Experiment shows that the exosome extraction method of the invention can realize highly-efficient extraction of the exosomes from the human body fluid or the cell culture medium, the extraction time is short, the purity of the obtained exosomes is high, complete exosomes can be obtained by eluent elution, and the used extraction materials can be recycled and reused; in addition, ext