Preparation method of zearalenone ratio fluorescence probe

The invention discloses a preparation method of a zearalenone ratio fluorescence probe. The method comprises the following steps: (1) preparation of a silicon quantum dot: APTES((3-aminopropyl)-triethoxysilane) and sodium citrate are subjected to a reaction in glycerol, and silicon quantum dots are obtained; (2) preparation of CdTe (cadmium telluride) quantum dots: tellurium powder, sodium borohydride and the like are subjected to a reaction, a NaHTe (sodium hydrogen telluride) precursor is obtained and added to the reaction solution, and red CdTe quantum dots are obtained; (3) preparation ofaptamer grafted silicon quantum dots: the prepared silicon quantum dots, N-(3-dimethylaminopropyl)-N-carbodiimide hydrochloride, N-hydroxysuccinimide, and a zearalenone aptamer are subjected to a reaction in an HEPES buffer solution, and silicon fluorescence quantum dots with surface grafted with the zearalenone aptamer are obtained; (4) synthesis of the ratio fluorescence probe: the red CdTe quantum dots are mixed with t