Method for constructing multi-point mutation nucleic acid sequence

The invention discloses a method for constructing a multi-point mutation nucleic acid sequence, and relates to the technical field of gene engineering. The method for constructing the multi-point mutation nucleic acid sequence utilizes restriction enzyme dependent on methylation, 5-methyl dCTP, and the like to achieve rapid construction of the multi-point mutation of a gene. The method can quicklyand conveniently construct the nucleic acid sequence with the multi-point mutation. 本发明公开了一种构建多点突变核酸序列的方法，涉及基因工程技术领域。本发明公开的构建多点突变核酸序列的方法利用依赖于甲基化的限制性内切酶和5-甲基dCTP等来达到快速构建基因多点突变。该方法可以快速、便捷地构建出具有多点突变核酸序列。