Protecting agent for stability of marine protease-producing bacillus

The invention relates to a protecting agent for the stability of a marine protease-producing bacillus, and belongs to the technical field of microorganisms. The protecting agent comprises a Tris-HCl buffer solution, glycerol, mannitol, EDTA, potassium chloride, magnesium sulfate, disodium hydrogen p...

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Bibliographische Detailangaben
Hauptverfasser: YAN XIN, WANG ZHENHUA, CHANG LIRONG, YAN LIN, DING JIANZI, YAO YANYAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a protecting agent for the stability of a marine protease-producing bacillus, and belongs to the technical field of microorganisms. The protecting agent comprises a Tris-HCl buffer solution, glycerol, mannitol, EDTA, potassium chloride, magnesium sulfate, disodium hydrogen phosphate and 2-ethylhexanoic acid diphenyl antimony, wherein the Tris-HCl buffer solution, glycerol,mannitol, EDTA, potassium chloride, magnesium sulfate, disodium hydrogen phosphate and 2-ethylhexanoic acid diphenyl antimony are prepared in a certain ratio into the liquid protecting agent. The protecting agent for the stability of the marine protease-producing bacillus solves the problems that a marine protease-producing bacillus strain is denatured under the low-temperature refrigeration condition, and the survival rate is low, and the survival rate of the strain can be stabilized at 95% or above. 本发明涉及一种海洋产蛋白酶芽孢杆菌稳定性保护剂,属于微生物技术领域。该保护剂包括Tris-HCl缓冲液、甘油、甘露醇、EDTA、氯化钾、硫酸镁、磷酸氢二钠、2-乙基己酸二苯基锑,所述的Tris-HCl缓冲液、甘油、甘露醇、EDTA、氯