Method for evaluating fungus sterilizing effect of disposable sanitary accessories by ATP biofluorescence lgC-lgI standard curve method

The invention relates to a method for evaluating fungus sterilizing effect of disposable sanitary accessories. The method comprises the detection steps of meeting sample extraction requirements; selecting equipment and preparing a reagent and a culture medium; performing strain preserving and activa...

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Bibliographische Detailangaben
Hauptverfasser: LI WENJIE, CUI ZONGYAN, LIAN SUMEI, LOU QIAOZHE, JIN HUIDA
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to a method for evaluating fungus sterilizing effect of disposable sanitary accessories. The method comprises the detection steps of meeting sample extraction requirements; selecting equipment and preparing a reagent and a culture medium; performing strain preserving and activating and preparing fungus suspension; after methylthioninium chloride staining, counting the content C of viable fungi in the fungus suspension through a hemocytometer; establishing a standard curve of the ATP concentration log lgC-relative fluorescence intensity log lgI and performing calibration on C of viable fungi in inoculated fungus liquid; performing neutralizer identification and sterilizing test; performing I determination and C reckoning on recovered liquid; calculating fungus sterilizing rate R and fungus sterilization index A; and performing result evaluation. The method has the characteristics that the fungus sterilizing effect of sanitary accessories, expressed by R or A, is subjected to pinpoint quan