TARGETED RECOMBINATION BETWEEN HOMOLOGOUS CHROMOSOMES AND USES THEREOF

Methods of targeted recombination between homologous chromosomes in the genome of a somatic plant cell are described herein, wherein the target site may be located within a region of euchromatin or a region of heterochromatin. These methods may be used to induce a somatic plant cell into using targeted recombination between homologous chromosomes leading to targeted crossover or gene conversion. Methods described utilize a preselected endogenous target site at a locus having polymorphic alleles on the homologous chromosomes. Target site loci disclosed include those within euchromatin and heterochromatin. 本文描述了体细胞植物细胞基因组中同源染色体之间靶向重组的方法，其中靶位点可位于常染色质区域或异染色质区域内。这些方法利用同源染色体之间的靶向重组，可用于诱导体细胞植物细胞，导致靶向交叉或基因转换。所描述的方法利用在同源染色体上具有多态性等位基因的基因座处的预选内源靶位点。公开的靶位点基因座包括常染色质和异染色质中的那些基因座。