Avian influenza virus electrochemical sensor based on silk-screen printing electrodes

he invention, which relates to the fields of biosensing and virus detection, specifically discloses an avian influenza virus electrochemical sensor based on silk-screen printing electrodes. On the basis of the silk-screen printing mode, a working electrode, a counter electrode, a reference electrode...

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Hauptverfasser: GAO JIN'E, CHEN JUNHUA
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:he invention, which relates to the fields of biosensing and virus detection, specifically discloses an avian influenza virus electrochemical sensor based on silk-screen printing electrodes. On the basis of the silk-screen printing mode, a working electrode, a counter electrode, a reference electrode, a guide rail and an insulating layer are printed on the surface of a printed substrate to make silk-screen printing electrodes. The working electrode among the silk-screen printing electrodes is modified with nanogold and HRP and an avian influenza virus electrochemical immunosensor is constructed by using the HRP as a carrier to fix an anti-H9N2 avian influenza virus hemagglutinin rabbit polyclonal antibody; signal amplification is carried out on the signal by using the carbon nanosphere-labeled glucose oxidase-labeled anti-H9N2 avian influenza virus hemagglutinin monoclonal antibody, so that the high-sensitivity measurement of the H9N2 avian influenza virus is realized. 本发明涉及生物传感领域、病毒检测领域,具体公开了一种基于丝网印刷电极的禽流感病毒电