Community dividing method for Dongxiang wild rice
The invention discloses a community dividing method for Dongxiang wild rice. The method comprises the following steps of selection of a test material; DNA extraction; design of a mitochondria marker; genotyping. The obtained marker is designed for mitochondria variation sequences of different variet...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a community dividing method for Dongxiang wild rice. The method comprises the following steps of selection of a test material; DNA extraction; design of a mitochondria marker; genotyping. The obtained marker is designed for mitochondria variation sequences of different varieties, the specificity is high, so that during detection, it is unnecessary to separately extract mitochondria DNA for PCR amplification, and only total DNA is needed as a template for detection; according to the method, the marker is the mitochondria molecular marker, molecular identification can be conducted on Dongxiang wild rice communities from the point of maternal inheritance, and differentiation of the Dongxiang wild rice communities is known; the marker is utilized for detecting the Dongxiang wild rice communities, not only is the cost reduced, but also the genetic structure and distribution information of the Dongxiang wild rice communities can be accurately acquired, and accurate protection and utilization |
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