Method for extracting small-molecule active peptide from pearl powder through complex enzyme

The invention discloses a method for extracting a small-molecule active peptide from pearl powder through a complex enzyme. The method comprises the steps of adding the pearl powder into an acid solution for acidolysis, and collecting liquid supernatant and first insoluble protein; brewing the first...

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Bibliographische Detailangaben
Hauptverfasser: WEI DILAN, TANG CHANGMING, YANG GUICHANG, SI GUANGJIE, WANG TING, ZHOU MUYAN, RUAN HUAJUN, HE QINGQING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for extracting a small-molecule active peptide from pearl powder through a complex enzyme. The method comprises the steps of adding the pearl powder into an acid solution for acidolysis, and collecting liquid supernatant and first insoluble protein; brewing the first insoluble protein with water, and then conducting centrifugation to obtain second liquid supernatant and second insoluble protein; merging the first liquid supernatant and the second liquid supernatant; conducting vacuum hot concentration on the merged first liquid supernatant and second liquid supernatant, separating out calcium lactate, after filtrating, mixing the calcium lactate with the second insoluble protein, and conducting homogenation to prepare a homogenation liquid; utilizing a complex protease liquid for conducting enzymolysis on the homogenation liquid under a weak acid condition to obtain an enzymolysis liquid, wherein the complex enzymolysis liquid comprises a trypsin liquid, a papain liquid and a