Glutamine synthetase mutant with glufosinate resistance and application and cultivation method of glutamine synthese mutant

The invention discloses a glutamine synthetase mutant with glufosinate resistance and application and a cultivation method of the glutamine sythetase mutant, and relates to the field of biotechnology.Compared the amino acid sequence of the glutamine synthetase mutant with the reference sequence, the glutamine synthetase mutant with glufosinate resistance disclosed in the present invention has oneor two combinations of the following mutations that: (1) the mutation of the glutamine sythetase mutant corresponding to the 59th amino acid site of the reference sequence is X1, wherein X1=A, C, D,E, F, G, H, I, K, P, T, V or Y; and (2) the mutation of the glutamine sythetase mutant corresponding to the 296th amino acid site of the reference sequence is X2, and X2=A, D, E, G, I, K, M, P, Q, R,S, T or V. The glutamine synthetase mutant has the characteristic of glufosinate resistance, and can be used to cultivate new varieties of plants with glufosinate resistance. 本发明公开了具有草铵膦抗性的谷氨酰胺合成酶突变体及其应用和培育方法，涉及生物技术领域。本发明公开的具有草铵