Tumor cell three-dimensional model and construction method and application thereof

The invention belongs to the technical field of tumor cell three-dimensional culture, and particularly relates to a tumor cell three-dimensional model and a construction method and application thereof. According to the construction method, an alginate solution is added into a mixed cell suspension solution containing tumor cells and fibroblasts, a mixed cell-alginate solution is obtained, and thenthe mixed cell-alginate solution is dropped into a calcium salt solution to obtain mixed cell microspheres. The tumor cell three-dimensional model and the construction method and application thereofhave the advantages that alginate and calcium salt are adopted for cross-linking to prepare the microspheres as cytoskeletons, thereby reducing the cost of three-dimensional culture of the tumor cells, the alginate has good biocompatibility and non-immunogenicity, thereby being capable of providing a good three-dimensional growth environment for the cells, and three-dimensional co-culture is carried out on the tumor cells