Laboratory mouse adipose tissue paraffin section tissue processing method

The invention relates to a laboratory mouse adipose tissue paraffin section tissue processing method and belongs to the pathological tissue section manufacturing technology field. A traditional adipose tissue processing scheme takes a long time and is inefficient. In the invention, the above problem...

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Bibliographische Detailangaben
Hauptverfasser: YIN WENJUAN, JIAN XIAOTING, LI LINGMIN, QIAO AIXIU, FU CHAO, GUO LINZHI, WANG XIAOHUI, YUE DAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a laboratory mouse adipose tissue paraffin section tissue processing method and belongs to the pathological tissue section manufacturing technology field. A traditional adipose tissue processing scheme takes a long time and is inefficient. In the invention, the above problems are solved. The method comprises the following steps of S1, fixing laboratory mouse in vitro adipose tissues in a neutral buffered formalin solution with a concentration of 10% for 24 hours, and then taking out the in vitro adipose tissues for later use; and S2, successively using following gradient concentration ethanol solutions to dehydrate the in vitro adipose tissues, and acquiring the dehydrated in vitro adipose tissues which are used for subsequent embedding. Time for manufacturing a laboratory mouse adipose tissue paraffin section in the method of the invention is shortened by 16 hours compared with the time in a traditional program, and working efficiency is greatly improved. Thesection is complete witho