Purification method of agkistrodon acutus venom having oral squamous cell carcinoma resisting activity

The invention discloses a purification method of agkistrodon acutus venom having oral squamous cell carcinoma resisting activity. The purification method comprises the following operating steps of (1)dissolving agkistrodon acutus venom, performing centrifuging, performing DE-52 column chromatography, performing detection according to an ultraviolet detection 280nm spectrogram to obtain eluent having 7 peaks in total, loading the eluent having IV peak as a collection target into a dialysis bag, performing desalination in redistilled water, performing dehydration in PEG6000, performing freeze-drying to obtain a primary product I, and then performing next-step chromatography; (2) dissolving the primary product I, performing centrifuging, performing C-25 chromatography, detecting all the eluent of the target peak according to the ultraviolet detection 280nm spectrogram, performing loading into a dialysis bag, performing desalinating in redistilled water, performing dehydrating in PEG6000,performing freeze-drying