Method for detecting related substances in L-alpha-glycerophosphorylcholine

The invention relates to a method for detecting related substances in chemically synthesized L-alpha-glycerophosphorylcholine by liquid chromatography, wherein the used chromatographic column is a polar silica gel column or other equivalent chromatographic columns; the mobile phase is methanol-buffered saline solution, acetonitrile-buffered saline solution or methanol-acetonitrile-buffered salinesolution and contains additive. The method has simple operation, good specificity, precision and accuracy, and good durability, and is suitable for detection and quality control of related substancesin chemically synthesized L-alpha-glycerophosphorylcholine. 本发明涉及一种液相色谱法检测化学合成的L-α-甘油磷酰胆碱中有关物质检查方法。其中所使用的色谱柱为极性硅胶柱或其他等效色谱柱；流动相为甲醇-缓冲盐溶液、乙腈-缓冲盐溶液或者甲醇-乙腈-缓冲盐溶液，并包含了添加剂。该方法操作简便，专属性、精密度和准确度较好，方法的耐用性良好，适用于化学合成的L-α-甘油磷酰胆碱中有关物质的检查和质量控制。