Primer group and kit for detecting rice nitrate transport protein gene, and detection method and application thereof

The invention provides a primer group and kit for detecting a rice nitrate transport protein gene NRT1.1B, and a detection method and an application thereof, and relates to the technical field of ricebreeding. The primer group includes forward primers, reverse primers and general primers. Specific SNP loci for detecting the rice nitrate transport protein gene NRT1.1B can be screened by the primergroup, and the identification accuracy and detection efficiency of nitrogen-efficient germplasm resources are significantly improved; the detection method is accurate, reliable, and easy to operate,reduces the scale and frequency of determination of the nitrogen utilization rate, reduces the research and development costs, shortens breeding process and improves breeding efficiency. In the fieldof molecular assisted breeding, a technical support is provided for the creation of the nitrogen-efficient germplasm resources and cultivation of new nitrogen-efficient rice varieties. 本发明提供了检测水稻硝酸盐转运蛋白基因NRT1.1B的引物组、试剂盒及其检测方法和应用