Method for simultaneously positioning multiple genes

The invention relates to a method for simultaneously positioning multiple genes. The method comprises the steps of creating a BC1F1 group and an F2 group, screening a codominant polymorphic molecule mark, performing genotyping on the F2 group by means of the molecule mark, then classifying the materials with the same genotype to one group, investigating the phenotype difference between different groups and performing statistical analysis, performing verification by means of a hybrid genotype F2 single plant progeny, namely positioning a certain phenotype trait gene next to the specific molecule mark. The method of the invention has advantages of the positioning the quantitative character and qualitative character, improving group utilization efficiency and realizing large-scale gene positioning by emans of a phenomics-related facility. 本发明涉及一种同时定位多个基因的方法，具体是构建BC1F1群体和F2群体，筛选共显性的多态性分子标记，利用分子标记对F2群体进行基因分型，然后把相同基因型的材料分成一组，调查不同分组之间的表型差异并进行统计分析，使用杂合基因型的F2单株后代进行验证，即可将某一表型性状基因定位在特定的分子标记附近。本发明可以对数量性状和质量性状进行定位，构建的群体可以