Preparation method of mouse anti-human SAA monoclonal antibody

The invention belongs to the technical field of monoclonal antibodies and particularly relates to a preparation method of a mouse anti-human SAA monoclonal antibody. The method comprises the followingsteps of (1) antigen preparation; (2) animal immunization; (3) cell fusion, wherein preparation work including preparation of spleen cells and preparation of a feeding layer is carried out before fusion; (4) screening and cloning of hybridoma cells; (5) expanded production of the monoclonal antibody; (6) determination of the type, subtype and ascites titer of the monoclonal antibody. In step 2, amulti-point injection mode is adopted during first immunization and second immunization, and an emulsified antigen is used and injected into the intradermal cells, foot pads and the abdominal cavityseparately; at the same time, shock immunization is performed once three days before fusion. According to the preparation method, a multi-point combined immunization mode and a shock immunization modeare adopted, the antigen do