MODIFIED MULTIPLEX AND MULTISTEP AMPLIFICATION REACTIONS AND REAGENTS THEREFOR

The invention relates to reagents and methods for improving the efficiency of multiplex nucleic acid amplification, in particular where overlapping amplicons are to be generated. The invention also relates to reagents and methods for improving the efficiency of multistep nucleic acid amplification, in particular the performance of two separate amplification reactions designed to occur in sequencein the same reaction mixture or vessel. The invention further relates to reagents and methods for improving multistep nucleic acid amplification reactions by controlling the output of the first amplification reaction. In particular, primers are provided that minimise the formation of aberrant amplification products. Such primers are particularly useful where first and second amplification reactions take place in a single reaction mixture or vessel. 本发明涉及用于提高多重核酸扩增效率的试剂和方法，特别是在打算产生重叠扩增子的情况下。本发明还涉及用于提高多步核酸扩增效率的试剂和方法，特别是被设计成在同一反应混合物或容器中按顺序发生的两个单独扩增反应的执行。本发明进一步涉及用于通过控制第一扩增反应的输出来改善多步核酸扩增反应的试剂和方法。特别地，提供了一些引物以减少异常扩增产物的形成。当第一