Avirulent C-type clostridium botulinum gene engineering subunit vaccine and production method thereof

The invention relates to an avirulent C-type clostridium botulinum gene engineering subunit vaccine and a production method thereof. The prepared avirulent C-type clostridium botulinum subunit vaccineis produced by adopting recombinant protein (rMBP-BoHC), which is subjected to codon optimization and contains a maltose-binding protein (MBP) tag, of an avirulent area HC (BoHC) of a clostridium botulinum toxin (BoNTs) heavy chain, so that not only is the safety of the vaccine guaranteed furthest, but also the effectiveness of the vaccine is maintained. Meanwhile, an antigen of the vaccine can be expressed in a soluble mode, so that the avirulent C-type clostridium botulinum gene engineering subunit vaccine has the advantages of being simple in preparation technology, low in immunizing dose,good in immune efficacy and the like, and is an ideal candidate vaccine for upgrading and updating current C-type clostridium botulinum vaccines of the nation. 本发明涉及一种无毒C型肉毒梭菌基因工程亚单位疫苗及其生产方法，本发明制备的无毒性C型肉毒梭菌亚单位疫苗，系采用经过密码子优化、含有