Recombinant rabies virus rHEP-Delta G-EGFP and application thereof
The invention relates to the technical field of antibody detection, in particular to recombinant rabies virus rHEP-delta G-EGFP and an application thereof. The recombinant rabies virus rHEP-delta G-EGFP is a glycoprotein gene-deleted recombinant strain. The glycoprotein gene-deleted recombinant stra...
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Zusammenfassung: | The invention relates to the technical field of antibody detection, in particular to recombinant rabies virus rHEP-delta G-EGFP and an application thereof. The recombinant rabies virus rHEP-delta G-EGFP is a glycoprotein gene-deleted recombinant strain. The glycoprotein gene-deleted recombinant strain has the following characteristics: a) the strain contains cell-derived standard virulent strain glycoprotein; b) the strain is capable of infecting cells and expressing a green fluorescent protein in the cells; and c) the strain is unable to self-replicate and diffuse in cells and animal bodies.A method for detecting a rabies virus neutralizing antibody level established by using the recombinant rabies virus rHEP-Delta G-EGFP has the following advantages: firstly, the virus cannot be passaged and propagated in cells and living bodies, so that the virus is safer; secondly, the detection method performs observation under a direct fluorescence microscope and is more economical and convenient; and thirdly, the detec |
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