Nucleic acid polypeptide complex probe and preparation method and application thereof

The invention relates to a nucleic acid polypeptide complex probe and a preparation method and an application thereof. According to the invention, a structure is shown in the specification: the R1 isDNA: 5'-biotin-TCCATCATTACCCGGCAGTATTA-3'; and R2 is a substrate polypeptide: GDKAVLGVDPFR. The method combines a double-chain specific nuclease-mediated isothermal amplification strategy with a targeted proteomic technology for the first time, selectively degrades a DNA chain in a DNA chain of a DNA-polypeptide: miRNA hybrid by the double-chain specific nuclease, combines the cycle amplificationability to release a large number of nucleic acid polypeptide complex probe fragments with a very small amount of miRNAs with the high sensitivity, high selectivity and wide dynamic range of the polypeptide targeting a proteomics detection report, and develops a novel quasi-targeted proteomics method to quantifying low-abundance miRNAs, such as miRNAs in stem cells. 核酸多肽复合物探针及其制备方法和应用，结构如下所示：所述R1为DNA：5'-biotin-TCCATCATTACC