CELL CULTURE MEDIUM, AND CELL CULTURE APPARATUS AND CELL CULTURE METHOD EACH USING SAME

A cell culture apparatus (1) according to the present invention is characterized by being provided with: a culture vessel (2) in which a cell culture medium is stored to culture cells, wherein at least one of culture medium components in the cell culture medium has the form of a conjugate with a sti...

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Bibliographische Detailangaben
Hauptverfasser: SHIBUYA KEISUKE, KONDOU TAKEYUKI, MARUYAMA MASASHI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:A cell culture apparatus (1) according to the present invention is characterized by being provided with: a culture vessel (2) in which a cell culture medium is stored to culture cells, wherein at least one of culture medium components in the cell culture medium has the form of a conjugate with a stimulus-responsive polymer; a stimulus application mechanism (4) for applying a predetermined stimulusto the conjugate to cause a certain change in response of the stimulus-responsive polymer; and a separation mechanism (5) for retaining the conjugate in the cell culture medium on the basis of the property developed in the stimulus-responsive polymer due to the change in response, and separating at least a portion of the culture medium components other than the conjugate from the cell culture medium. 本发明的细胞培养装置(1)的特征在于,具备:培养槽(2),其贮存细胞培养用培养基来培养细胞,所述细胞培养用培养基的培养基成分中的至少1种由与刺激响应性高分子结合的结合物构成;刺激赋予机构(4),其向所述结合物赋予特定的刺激、使所述刺激响应性高分子产生特定的响应变化;和分离机构(5),其基于因所述响应变化而在所述刺激响应性高分子中出现的性状将所述结合物留在所述细胞培养用培养基中,将除所述结合物外的其他所述培养基成分中的至少一部分从所述细胞