Tea tree sucrose synthase CsSUS587, preparation method and application

The invention belongs to the field of the molecular biological technique, and particularly relates to tea tree sucrose synthase CsSUS587, a preparation method and application. Tea tree cDNA serves asa template, a primer is designed, and a target gene is subjected to PCR amplification; a connecting system is established, reacting is carried out, and an expression carrier is obtained; the established carrier is converted into competent cells; the tea tree sucrose synthase is separated and purified. The prepared tea tree sucrose synthase CsSUS587 is high in purity, it can be promoted that uridine diphosphate glucose capable of being used for glucoside synthesis is generated by catalytic uridine diphosphate and sucrose, the uridine diphosphate glucose and glycosyltransferase are combined to be used, and the synthesis efficiency of corresponding glucoside can be improved. 本发明属于分子生物技术领域，特别涉及一种茶树蔗糖合酶CsSUS587、制备方法及应用，包括以茶树cDNA为模板，设计引物，PCR扩增目的基因；构建连接体系反应得表达载体；将构建载体转化至感受态细胞中；茶树蔗糖合酶的分离纯化。本发明从制备的蔗糖合酶CsSUS587纯度高，能促进可催化尿苷二