Nucleotide sequence tWPRE and gene expression vector and application thereof

The invention belongs to the field of genetic engineering and particularly relates to a nucleotide sequence tWPRE including a WPRE-polyA element and gene expression vector and application thereof. Thenucleotide sequence tWPRE including the WPRE-polyA element has the nucleotide sequence shown in SEQ ID NO:1. The gene expression vector comprises the nucleotide sequence tWPRE. The invention furtherimproves the WPRE-polyA element. The optimized tWPRE is obtained, the effectiveness of the tWPRE element is verified on the aspects of plasmid transfection and virus infection, the high-efficiency transgene expression efficiency and virus infection efficiency are guaranteed while the capacity of the vector is expanded, more specific promoters and genes can be carried by an AAV vector, the action range of an AAV virus is expanded, and a new more specific and efficient expression tool is provided for basic scientific research and clinical research. 本发明属于基因工程领域，更具体地说本发明涉及一种含有WPRE-polyA元件的核苷酸序列tWPRE及其基因表达载体和应用。所述含有WPRE-pol