CROSS-TALK COMPENSATION

Method and system for compensating intensity biases in a plurality of digital images. Each digital image of the plurality of digital images contains a plurality of objects and each of the plurality ofobjects is configured to receive at least one molecule comprising genetic information, wherein the at least one molecule is configured to receive one of at least a first fluorescent compound and a second fluorescent compound. A first digital image of the plurality of digital images is taken by an optical imaging system during emission of electromagnetic radiation by the first fluorescent compound, and a second digital image of the plurality of digital images is taken by the optical imaging system during emission of electromagnetic radiation by the second fluorescent compound. 本发明涉及用于在多个数字图像中补偿强度偏倚的方法和系统。所述多个数字图像中的每个数字图像含有多个对象，并且所述多个对象中的每一个被配置成接收包含遗传信息的至少一个分子，其中所述至少一个分子被配置成接收至少第一荧光化合物和第二荧光化合物中的一者。所述多个数字图像中的第一数字图像在所述第一荧光化合物的电磁辐射发射期间通过光学成像系统获取，并且所述多个数字图像中的第二数字图像在所述第二荧光化合物的电磁辐射发射期间通过所述光学成像系统获取。