HPLC-ELSD detection method of gentamicin C component content in gentamicin procaine vitamin B12 capsules

The invention belongs to the chemical detection field and especially relates to an HPLC-ELSD detection method of a gentamicin C component content in gentamicin procaine vitamin B12 capsules. The method comprises the following steps of (1) carrying out a chromatographic condition and system suitabili...

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Hauptverfasser: ZHU RONG, DENG MING, ZHU JIANPING, CHEN HAN, HUANG LILI, TAN JUYING, LU RIGANG, ZHAO ZHUANG
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creator ZHU RONG
DENG MING
ZHU JIANPING
CHEN HAN
HUANG LILI
TAN JUYING
LU RIGANG
ZHAO ZHUANG
description The invention belongs to the chemical detection field and especially relates to an HPLC-ELSD detection method of a gentamicin C component content in gentamicin procaine vitamin B12 capsules. The method comprises the following steps of (1) carrying out a chromatographic condition and system suitability test; (2) preparing a solution; and (3) testing and the like. Operation of the method is simple,detection efficiency is high, an error is small, and the detection method is stable; accuracy, precision, specificity, a detection limit, and durability and the like of the method accord with a methodological verification requirement; high practicality is achieved; and the gentamicin C component content in the gentamicin procaine vitamin B12 capsules can be rapidly detected so as to effectively control the quality of the gentamicin procaine vitamin B12 capsules. 本发明属于化学检测领域,具体涉及一种庆大霉素普鲁卡因维B12胶囊中庆大霉素C组分含量的HPLC-ELSD检测方法,包括(1)色谱条件与系统适用性试验(2)溶液制备(3)测定等步骤。该方法操作简单,检测效率高,误差小,检验方法较稳定,该方法的准确度、精密度、专属性、检测限、耐用性等均符合方法学验证要求,具有很强的实用
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title HPLC-ELSD detection method of gentamicin C component content in gentamicin procaine vitamin B12 capsules
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