HPLC-ELSD detection method of gentamicin C component content in gentamicin procaine vitamin B12 capsules

The invention belongs to the chemical detection field and especially relates to an HPLC-ELSD detection method of a gentamicin C component content in gentamicin procaine vitamin B12 capsules. The method comprises the following steps of (1) carrying out a chromatographic condition and system suitability test; (2) preparing a solution; and (3) testing and the like. Operation of the method is simple,detection efficiency is high, an error is small, and the detection method is stable; accuracy, precision, specificity, a detection limit, and durability and the like of the method accord with a methodological verification requirement; high practicality is achieved; and the gentamicin C component content in the gentamicin procaine vitamin B12 capsules can be rapidly detected so as to effectively control the quality of the gentamicin procaine vitamin B12 capsules. 本发明属于化学检测领域，具体涉及一种庆大霉素普鲁卡因维B12胶囊中庆大霉素C组分含量的HPLC-ELSD检测方法，包括（1）色谱条件与系统适用性试验（2）溶液制备（3）测定等步骤。该方法操作简单，检测效率高，误差小，检验方法较稳定，该方法的准确度、精密度、专属性、检测限、耐用性等均符合方法学验证要求，具有很强的实用