Method for preparing ganoderma lucidum strains
The invention discloses a method for preparing ganoderma lucidum strains. The method comprises the following preparation steps of (1) preparation of a culture medium, wherein the culture medium comprises, by weight, 200 g of potatoes, 20 g of agar, 20 g of glucose, 2 g of peptone, 2 g of multivitami...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a method for preparing ganoderma lucidum strains. The method comprises the following preparation steps of (1) preparation of a culture medium, wherein the culture medium comprises, by weight, 200 g of potatoes, 20 g of agar, 20 g of glucose, 2 g of peptone, 2 g of multivitamins, 2 g of potassium dihydrogen phosphate, 0.5 g of magnesium sulfate and 1000 ml of distilled water; (2) preparation of the strains, wherein preparation of the strains includes the following preparation steps that a, fresh ganoderma lucidum is selected for tissue separation; b, seeds are taken; c,inoculation blocks are taken and inoculated into a test tube culture medium; d, the inoculation blocks are placed in a biochemical culture box for culture to obtain mother species; e, wood chips and the like are taken and placed in a bottle, and the mother species are inoculated into the bottle and grow for 45 days to obtain original species; f, the wood chips and the like are taken and placed inanother bottle, and the or |
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