Method for producing allergenic component-free purified bee venom, and allergenic component-free purified bee venom produced thereby

The present invention relates to a method for producing an allergenic component-free purified bee venom, and an allergenic component-free purified bee venom produced thereby, the method enabling the production of an allergenic component-free purified bee venom by comprising the steps of: (a) dilutin...

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Hauptverfasser: LEE JAE-SEOK, JUNG TAE-KGEUN, KIM DAE-KYUM, UM JAE-YEON, YE HYUN-OK, PARK HYO-JIN, NA SUNG-HOON, KIM HYE-SOO, PARK EUN-YOUNG, LEE SOO-HYEONG, SIM TAE-KYUNG, CHO EUN-SUK, KIM SUNG-KYUN, KIM JONG-HYUN, CHOI WON-HOI, JUNG YOUNG-JIN, KIM SE-RAN, KIM WAN-YEONG, SHIN DONG-HA, BAEK YOUNG-MEE, KIM DAN-YOUNG, SONG YOUNG-GIL, CHOI SEONG-HWAN, JUNG YOUNG-MOK, KO HWAN-JOO, JEON YONG-WOO, KIM YONG-SOO
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The present invention relates to a method for producing an allergenic component-free purified bee venom, and an allergenic component-free purified bee venom produced thereby, the method enabling the production of an allergenic component-free purified bee venom by comprising the steps of: (a) diluting a bee venom by adding a buffer solution thereto; (b) adding a protease to the diluted solution diluted in step (a), and then having same undergo an enzymatic reaction; (c) coagulating a protein of the reactant of step (b) by adding cold ethanol to the reactant; (d) precipitating the coagulated material by centrifuging the coagulated reactant of step (c); and (e) obtaining a supernatant from the reactant of step (d) in which the coagulated material is precipitated, then drying same. 本发明涉及制备去除过敏成分的精制蜂毒的方法及通过所述方法制备的去除过敏成分的精制蜂毒,其特征在于,所述方法包括以下步骤:(a)向蜂毒中添加缓冲溶液进行稀释;(b)向所述步骤(a)的经过稀释的稀释液中添加蛋白酶后进行酶反应;(c)在所述步骤(b)的反应物中加入经过冷却的乙醇,从而凝固反应物的蛋白质;(d)将所述步骤(c)的经过凝固的反应物进行离心分离,从而使凝固物沉淀;以及(e)取所述步骤(d)的凝固物沉淀的反应物中的上清液后进行干燥。