Homogeneous phase chemiluminescence microfluidic chip and detection method thereof

The invention relates to a homogeneous phase chemiluminescence microfluidic chip and a detection method thereof. The invention combines the spatial proximity chemiluminescence technology with the microfluidic chip technology; and the used reagents include an enzyme label, a luminescent label, a triggering adjuvant, a triggering agent and a calibrator; the enzyme label, the luminescent label and the triggering adjuvant are embedded in the microfluidic chip by using cystic dropping-embedding or freeze-drying technology; the enzyme label contains an HRP-labeled IL-6 monoclonal antibody and a phosphate buffer; the luminescent label contains 9,10-dihydroacridine-labeled IL-6 monoclonal antibody and a Tris buffer; the triggering adjuvant contains a luminescent adjuvant and a citrate buffer; thetriggering agent is a Tris-HCl buffer; and the calibrator contains an IL-6 calibrator and a phosphate buffer. The homogeneous phase chemiluminescence microfluidic chip can be effectively used for detecting antigens or antibod