Method for identifying purity of cucumis melo hybrid Tian No. 9 seeds based on EST-SSR markers

The invention discloses a method for identifying the purity of cucumis melo variety "Tian No. 9" hybrid seeds based on EST-SSR markers. According to the method, cucumis melo variety "Tian No. 9" hybrid seeds and biparental genome DNA thereof are used as a formwork, a cucumis melo EST sequence is published on a Gene Bank database, Primer 3.0 on-line primers are used for designing, and EST-SSR primers are designed. Through screening, a pair of complementary banding pattern primers of which the hybrid-variety banding pattern is biparental is obtained; through field verification experiment, the primers are good in stability and cooperate with the field experiment result, and the purity of the cucumis melo hybrid varieties can be identified. Through the adoption of the detection method disclosed by the invention, the seed purity identifying work can be completed within 3h, and the method has the advantages of being quick, low in cost, convenient to operate and the like. 本发明公开了一种基于EST-SSR标记的甜瓜品种"甜9号"杂交种种子纯度鉴定的方法。该方