Method for identifying purity of melon hybrid Jintian-100 seeds based on EST-SSR marker

The invention discloses a method for identifying purity of melon hybrid Jintian-100 seeds based on EST-SSR marker. The method takes genome DNA of a melon variety Jintian-100 hybrid and parents of themelon variety as a template, adopts a melon EST sequence published in the Gene Bank database, utilizes Primer 3.0 on-line primer design, and designs an EST-SSR primer. Through screening, a pair of primers whose hybrid band patterns are complementary band patterns of parents is obtained. Field verification tests show that the primers have good stability and are in good agreement with field test results, so that the purity identification of the melon hybrid variety can be carried out. The detection method disclosed by the invention can complete seed purity identification within 3h, and has the advantages of rapidness, low cost and convenient operation. 本发明公开了一种基于EST-SSR标记的甜瓜品种"津甜100"杂交种种子纯度鉴定的方法。该方法以甜瓜品种"津甜100"杂交种及其双亲的基因组DNA为模板，通过Gene Bank数据库上公布甜瓜EST序列，利用Primer 3.0在线引物设计，设计EST-SSR引物。经过筛选，得到1对杂交种带型为双亲的互补带型引物，经田间验证试验引