Method for manufacturing natural-killer-t (NKT)-cell stimulating dendritic cell and method for manufacturing cell composition containing NKT-cell stimulating dendritic cell and NKT cell

Provided is a method for manufacturing NKT-cell stimulating dendritic cells, the method including: a step for placing mononuclear cells in a culturing vessel and allowing some of the mononuclear cellsto settle on a bottom surface of the vessel by keeping the culture still; a step for removing floating cells other than the cells that have settled on the bottom surface of the culturing vessel; a step for causing monocytes among the cells that have settled on the bottom surface to differentiate into immature dendritic cells by adding a predetermined factor to the culturing vessel; and a step forinducing mature dendritic cells into NKT-cell stimulating dendritic cells by adding [alpha]-galactosylceramide to the culturing vessel. 本申请提供了一种制备NKT细胞刺激性树突细胞的方法，该方法包括：将单个核细胞置于培养容器内并通过保持该培养容器静置而使得所述单个核细胞中的一些沉降于所述容器的底表面上的步骤；将除已沉降于所述培养容器的底表面上的细胞以外的悬浮细胞去除的步骤；通过向所述培养容器内添加预定的因子而使已沉降于所述底表面上的细胞中的单核细胞分化为未成熟树突细胞的步骤；通过向所述培养容器内添加预定的因子而使所述未成熟树突细胞成熟；和通过向所述培养容器内添加α-半乳糖神经酰胺而将成熟树突细胞诱导为NKT细胞刺激性树突细胞的步骤。