Method for long-term stabilization of inositol dehydrogenase, ketoamine oxidase and sphingomyelinase in liquid

The present invention provides a method for long-term stabilization of inositol dehydrogenase, ketoamine oxidase and sphingomyelinase in liquid. The method is used to prepare an enzymatic biochemicalkit for determining inositol, glycated albumin and small and dense low density lipoprotein cholesterol for a purpose of enabling the enzymatic biochemical kit to have a shelf life of 12 months or more. The method is characterized in that a composition of 4-formylbenzeneboronic acid and benzyldimethylphenol polyoxyethylene ether is added to a reagent solution for preserving enzymes, and concentrations of the 4-formylbenzeneboronic acid and benzyldimethylphenol polyoxyethylene ether are preferably 0.008-0.012 w/V% and 0.1-0.2 w/V%, respectively. 本发明提供一种使肌醇脱氢酶、酮胺氧化酶和鞘磷脂酶在液体中长期稳定的方法，用于配制测定肌醇、糖化白蛋白和小而密低密度脂蛋白胆固醇的酶法生化试剂盒，以达到使上述酶法生化试剂盒具有12个月以上有效期的目的。其特征在于，在保存酶的试剂溶液中添加4-甲酰基苯硼酸和苄基二甲基酚基聚氧乙烯醚的组合物，试剂中4-甲酰基苯硼酸和苄基二甲基酚基聚氧乙烯醚的浓度优选为0.008~0.012w/V%和0.1~0.2w/V%。