CAPS mark primer group for detecting non-genetically modified organism herbicide-resistant gene OsALS and application of CAPS mark primer group

The invention provides a CAPS mark primer group for detecting a non-genetically modified organism herbicide-resistant gene OsALS and application of the CAPS mark primer group, and relates to the technical field of rice breeding. The primer group comprises a forward primer OsALS-F and a reverse prime...

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Hauptverfasser: WANG JIAN, CHENG BAOSHAN, YUAN CAIYONG, WANG DI, LI GANG, LIN CAN, LI LEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a CAPS mark primer group for detecting a non-genetically modified organism herbicide-resistant gene OsALS and application of the CAPS mark primer group, and relates to the technical field of rice breeding. The primer group comprises a forward primer OsALS-F and a reverse primer R, wherein the nucleotide sequence of the forward primer OsALS-F is shown in SEQ ID NO.1 or SEQ IDNO.2, and the nucleotide sequence of the reverse primer R is shown in SEQ ID NO.3 or SEQ ID NO.4. The primer group can quickly and accurately select herbicide-resistant new rice varieties (lines) different in background, and the primer group has the advantages of being low in workload, low in production cost and the like. 本发明提供了一组检测水稻非转基因抗除草剂基因OsALS的CAPS标记引物组及其应用,涉及水稻育种技术领域。本发明所述引物组包括正向引物OsALS-F和反向引物R,所述正向引物OsALS-F的核苷酸序列如SEQ ID NO.1或SEQ ID NO.2所示,所述反向引物R的核苷酸序列如SEQ ID NO.3或SEQ ID NO.4所示。利用所述引物组可快速、准确选育出不同背景的抗除草剂水稻新品种(系),且还具有工作量小、生产成本低等优点。