Indirect ELISA detection kit for detecting avian adenovirus type 4 antibody by using Fiber2 protein

The invention discloses an avian adenovirus type 4 Fiber2 protein, a preparation method and a detection kit thereof.The avian adenovirus type 4 Fiber2 gene is connected with a pCold prokaryotic expression vector after codon optimization, BL21 competent cells are transformed, thallus is collected after induction expression, the thallus is subjected to ultrasonic disruption, centrifugation is carried out, supernatant is collected, and a His gel column is purified to obtain coating antigen.The kit comprises a Fiber2 protein-coated detection plate, a serum diluent, a goat anti-chicken HRP enzyme-labeled antibody with working concentration, a negative control, a positive control, a TMB color developing solution and a stop solution.The kit of the invention can rapidly and specifically detect thepresence or absence of the Fiber2 antibody in the serum, is used for detecting the adenovirus type 4 Fiber2 protein antibody in the chicken serum, has the advantages of good reaction specificity, high sensitivity, simple ope