Fermentation method for producing EPA (Eicosapentaenoic Acid) through mortierella alpina based on dissolved oxygen and pH (Potential of Hydrogen) regulation and control
The invention discloses a fermentation method for producing EPA (Eicosapentaenoic Acid) through mortierella alpina based on dissolved oxygen and pH (Potential of Hydrogen) regulation and control and belongs to the field of microorganism fermentation. The fermentation method disclosed by the inventio...
Gespeichert in:
Hauptverfasser: | , , , , , , |
---|---|
Format: | Patent |
Sprache: | chi ; eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The invention discloses a fermentation method for producing EPA (Eicosapentaenoic Acid) through mortierella alpina based on dissolved oxygen and pH (Potential of Hydrogen) regulation and control and belongs to the field of microorganism fermentation. The fermentation method disclosed by the invention adopts a suitable oxygen enriched air strategy to meet the dissolved oxygen requirements of thallus growth and product accumulation; the pH is regulated and controlled by utilizing ammonia water at a thallus growth period; meanwhile, the effect of a partial N source is also realized and rapid growth of thalli is accelerated; furthermore, the suitable pH is regulated and controlled by stages at a thallus growth stage and a lipid accumulation stage by adopting a staged pH regulation and controlstrategy, so that the yield of the EPA is further improved. A whole fermentation process adopts a fermentation manner of supplementing materials in batches; the initial glucose concentration is 30g/Land the concentration of r |
---|