Fusion type lipase higher in thermal stability and preparation method and application thereof

The invention belongs to the technical field of bioengineering, and discloses fusion type lipase higher in thermal stability and a preparation method and application thereof. The amino acid sequence of the fusion type lipase is shown in SEQ ID NO.4 or SEQ ID NO.5. The fusion type lipase is prepared from fusing a section of biparental oligopeptide composed of 30-32 amino acids with the N terminal in the amino acid sequence of the lipase for encoding thermomyces lanuginosus, and the amino acid sequence of the biparental oligopeptide is shown in SEQ ID NO.1 or SEQ ID NO.2. The two kinds of fusiontype lipase have desired heat-resistant characteristics, and therefore, the fusion type lipase is particularly suitable for industrial large-scaled production. 本发明属于生物工程技术领域，公开了一种热稳性提高的融合型脂肪酶及其制备方法和应用，融合型脂肪酶的氨基酸序列如SEQ ID NO.4或SEQ ID NO.5所示，融合型脂肪酶由编码梳棉状嗜热丝孢菌脂肪酶氨基酸序列中N端融合了一段由30～32个氨基酸组成的双亲短肽，所述的双亲短肽的氨基酸序列为SEQ ID NO.1或SEQ ID NO.2。两种融合型脂肪酶具有较理想耐热特性，因此特别适合工业化大规模生产。