Near infrared fluorescent probe capable of being used for responding to pH change in living cells in chronic wound development process and preparation method of probe

The invention discloses a near infrared fluorescent probe capable of being used for responding to the pH change in living cells in the chronic wound development process and a preparation method of theprobe, and belongs to the technical field of biological fluorescence analysis. The near infrared fluorescent probe is a near infrared cyanine probe based on a benzoyl hydrazine structure, and the general structural formula is shown in the description. A hydrazide group R3 or R4 serving as the group acting with the environment pH is utilized for construction through connection of benzoyl hydrazineand near infrared fluorophore (polymethine cyanines IR780) through an ether bond, and the near infrared fluorescence probe is obtained. The near infrared fluorescent probe can be used for bioimagingof changes of the endogenous PH and exogenous pH in living cells and the endogenous PH in the development process of chronic wounds on diabetic patients, the synthesis method is simple and easy to implement, and industrial popu