Fusion rhizopus chinensis lipase with thermal stability enhanced and application thereof

The invention belongs to the field of biotechnology, and discloses fusion rhizopus chinensis lipase with the thermal stability enhanced and application thereof. The amino acid sequence of the fusion rhizopus chinensis lipase is shown in SEQID NO.4 or SEQID NO.5. The fusion lipase is obtained by fusing a section of amphiphilic short peptide composed of 30-32 amino acids with the N terminal of the amino acid sequence encoding rhizopus chinensis lipase, and the amino acid sequence of the amphiphilic short peptide is shown in SEQID NO.2 or SEQID NO.3. The fusion lipase has ideal heat-resistant properties and thus is especially suitable for industrial mass production. 发明属于生物工程技术领域，公开了一种热稳性提高的融合型华根霉脂肪酶及其应用，所述的融合型脂肪酶的氨基酸序列如SEQ ID NO.4或SEQ ID NO.5所示，融合型脂肪酶由编码华根霉脂肪酶氨基酸序列中N端融合了一段由30～32个氨基酸组成的双亲短肽，所述的双亲短肽的氨基酸序列为SEQ ID NO.2或SEQ ID NO.3所示；两种融合型脂肪酶具有较理想耐热特性，因此特别适合工业化大规模生产。