Separation and purification and inoculating application method of rhododendron simiarum hance mycorrhiza fungus bacterial strain DPS-A

The invention discloses a separation and purification and inoculating application method of rhododendron simiarum hance mycorrhiza fungus bacterial strain DPS-A. The method comprises the following steps of (1) selecting mycorrhiza, and performing weighing; (2) sterilizing root system surfaces; (3) i...

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Hauptverfasser: LI YUELING, CHEN ZILIN, NI XIZHONG, LIU YA, ZHANG CENRONG, WANG PAN, CHEN JIANGFANG, BAO JINLIANG, ZONG KANKAN, ZHOU YUHONG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a separation and purification and inoculating application method of rhododendron simiarum hance mycorrhiza fungus bacterial strain DPS-A. The method comprises the following steps of (1) selecting mycorrhiza, and performing weighing; (2) sterilizing root system surfaces; (3) inspecting sterilization effects; (4) homogenizing root system tissue; (5) performing bacterial strain purification; (6)performing preliminary screening on bacterial strains; (7) preparing microbial inoculum; (8) performing inoculating with the bacterial strains; and (9) detecting the inoculating effects. The separation and purification and inoculating application method has the advantages that the bacterial strain separation and purification cycle is short, the technical operation is simple andconvenient, and high efficiency and scaled operation of rhododendron simiarum hance mycorrhiza fungus separation and purification are realized. 本发明公开了一种猴头杜鹃菌根真菌菌株DPS-A的分离纯化及其接种应用方法,包括以下步骤:(1)选菌根与称量;(2)根系表面灭菌;(3)检验灭菌效果;(4)根系组织