Preparation method for nattokinase and natto antimicrobial peptide mixed preparation, and applications

The invention provides a preparation method for a nattokinase and natto antimicrobial peptide mixed preparation, and belongs to the technical field of biomedicines. The method includes the following steps: 1) mixing natto and water, and performing grinding and filtering to obtain a grinding filtrate; 2) adding solid ammonium sulfate in the grinding filtrate, performing standing to get first supernatant, and performing disinfection so that a natto antimicrobial peptide solution can be obtained; 3) mixing the natto and phosphate buffer, performing grinding, mixing an obtained grinding object andthe phosphate buffer, and performing extraction and centrifugation to get second supernatant; 4) adding an ammonium sulfate aqueous solution in the second supernatant to perform mixing, and performing standing and centrifugation to get sediment so that nattokinase can be obtained; and 5) mixing the natto antimicrobial peptide solution and the nattokinase, performing filtration sterilization, andadjusting a pH value to 7.