METHOD FOR PRODUCING ALLERGENIC COMPONENT-FREE PURIFIED BEE VENOM USING YOLK, AND ALLERGENIC COMPONENT-FREE PURIFIED BEE VENOM PRODUCED THEREBY

The present invention relates to a method for producing an allergenic component-free purified bee venom, and an allergenic component-free purified bee venom produced thereby, the method enabling the production of an allergenic component-free purified bee venom by comprising the steps of: (a) preparing a buffer solution containing yolk; (b) adding a bee venom to the buffer solution prepared in step(a), and then stirring same; (c) coagulating a protein of the stirred reactant of step (b) by cooling the reactant while adding cold ethanol thereto; (d) precipitating the coagulated material by centrifuging the coagulated reactant of step (c); and (e) obtaining a supernatant from the reactant of step (d) in which the coagulated material is precipitated, and concentrating and drying same. 本发明涉及制备去除过敏成分的精制蜂毒的方法及通过所述方法制备的去除过敏成分的精制蜂毒，其特征在于，所述方法包括以下步骤：(a)准备包含卵黄的缓冲溶液；(b)向所述步骤(a)中所准备的缓冲溶液中添加蜂毒后进行搅拌；(c)将所述步骤(b)的经过搅拌的反应物进行冷却的同时，加入经过冷却的乙醇，从而凝固反应物的蛋白质；(d)将所述步骤(c)的经过凝固的反应物进行离心分离，从而使凝固物沉淀；以及(e)取所述步骤(d)的凝固物沉淀的反应物中的上清液，并进行浓缩及干燥。