Heparin detection kit and application thereof

The invention, which relates to the technical field of anticoagulant effect detection, discloses a heparin detection kit and application thereof. To be specific, the heparin detection kit comprises aheparin detection reagent including heparinase I being a liquid enzyme preparation; for long-time preservation, the heparinase I needs to be frozen and dried and then the processed heparinase I becomes solid one for preservation; however, after a certain amount of anhydrous calcium chloride, glycerol and D-trehalose that are used as protective agents are added into the heparinase I, the detectionreagent exists stably for long time, so that the long-time preservation requirement is met. With the heparin detection kit, the anticoagulant activity of heparin can be neutralized rapidly and specifically without affecting other blood components of blood coagulation, so that the residual situation of the heparin sodium salt and the anticoagulant effect of heparin sodium salt can be evaluated rapidly and precisely and thus