Method for quickly detecting pathogenic bacteria in cow endometritis

The invention discloses a method for quickly detecting pathogenic bacteria in cow endometritis. The method comprises the following steps of (1) extracting DNA of 6 common pathogenic bacteria which arecultured over night, in womb secretion by a reagent kit method; (2) in a reaction system containing the DNA of the bacteria, adding a PCR primer system which can detect the specificity sequence of the 6 kinds of bacteria at the same time, and performing amplification; and (3) preparing 2% of agarose gel, performing gelatin electrophoresis, wherein the voltage is 120 V and the time is 45 min, andthen performing electrophoresis ultraviolet imaging. Based on a multiple polymerase chain reaction technique, the method disclosed by the invention can realize detection of 6 kinds of the bacteria, including E.coli, S.aureus, T.pyogenes, S.uberis, P.mirabilis and P.aeruginosa. Compared with sequencing, techniques of substance PCR and the like are simpler and more convenient to operate, lower in cost, higher in sensitivity