Reagent kit for determining remnant-like particle cholesterol

The invention relates to a reagent kit for determining remnant-like particle cholesterol, and belongs to the technical field of determination of the remnant-like particle cholesterol. The reagent kitdisclosed by the invention comprises a reagent 1 and a reagent 2, wherein the reagent 1 comprises a P...

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Bibliographische Detailangaben
Hauptverfasser: FANG CHAOJUN, ZHENG JIANGPING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a reagent kit for determining remnant-like particle cholesterol, and belongs to the technical field of determination of the remnant-like particle cholesterol. The reagent kitdisclosed by the invention comprises a reagent 1 and a reagent 2, wherein the reagent 1 comprises a PIPES buffered solution, a color development substrate, a surfactant 1 and peroxidase; and the reagent 2 comprises a PIPES buffered solution, 4-aminoantipyrine, phospholipase D, cholesterol esterase, cholesterol oxidase, peroxidase and a surfactant 2. The reagent kit provided by the invention is simple and convenient to operate, suitable for clinical detection, wide in reagent detection linear scope, high in detection sensitivity, good in stability, high in anti-interference capacity, and good in accuracy and precision. 本发明涉及一种测定脂蛋白残粒胆固醇的试剂盒,属于脂蛋白残粒胆固醇测定技术领域。本发明所述试剂盒包括试剂1和试剂2;所述试剂1包括:PIPES缓冲液、显色底物、表面活性剂1和过氧化物酶;所述试剂2包括:PIPES缓冲液、4-氨基安替比林、磷脂酶D、胆固醇酯酶、胆固醇氧化酶、过氧化物酶和表面活性剂2。本发明提供的试剂盒操作简便,适用于临床检测,试剂检测线性范围宽,检测灵敏度高,稳定性好,抗干扰能力强