Method for determining content of polystyrene microspheres under environmental experiment simulation conditions

The invention belongs to the technical field of experimental detection. The invention relates to a method for determining the content of polystyrene (PS) microspheres under environmental experiment simulation conditions. The method mainly comprises the following steps: diluting PS microsphere turbid liquid by multiple ratios, adding the diluted and uniformly mixed turbid liquid into counting areasof a blood cell counting plate; letting the liquid stand in a wet box, photographing and numbering the plurality of counting areas under corresponding objective lenses, performing counting by using ImageJ image analysis software, and finally carrying out stability and significance analysis on data. According to the method, the shape and the content of microplastics can be visually displayed, accurate qualitative and quantitative analysis can be achieved based on a blood cell counting plate method and the ImageJ software, and the method is good in repeatability and high in stability and can effectively achieve the pur